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The single-learner CBT module can be run using standard web-browsers (e.g. Netscape).
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PCR was run using standard Taq (Invitrogen Corp).
Reactions were run using standard protocols and an ABI 7900HT Real-time PCR System (Applied Biosystems).
Otherwise, GATK was run using standard parameters according to GATK Best Practices recommendations [ 32, 33].
RepeatMasker was run using standard settings on the entire genome, with PIER 2.0 as a repeat library (Wegrzyn et al. 2013).
The tool is available in two versions with identical interface and features as a web service that can be run using any standard browser and as an offline application.
Reactions were run using the standard conditions on an ABI 7900HT Fast Real-Time PCR machine.
PCR reactions were run using the standard ABI program, 50°C for 2 min, 95°C for 10 min followed by 40 cycles of 94°C for 15 sec and 60°C for 1 min. PCR reactions contained both an experimental primer probe set as well as the reference primer-probe set for gapdh multiplexed in the same reaction [47].
A dissociation curve analysis was run using the standard protocol on the instrument.
All public methods were run using the standard defaults for eukaryotic protein prediction.
The programs were run using the standard settings for colorspace as recommended in the manual.
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