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Applications that require such hardware should be run on native systems.
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Purified samples were run on native PAGE gel with negative control (Fig. 2).
Samples were run on native 5% acrylamide gels for 1.5 hours at 250 V. Gels were subsequently fixed for 15 mins (acetic acid/methanol/water (10∶30∶60), dried and imaged using an instant imager (Packard).
To examine the possibility that optineurin can form oligomers or complexes, lysates from RGC5 cells were run on native blue gels followed by Western blotting for the endogenous optineurin under non-reducing conditions.
Either purified proteasomes or cell extracts were run on native gels.
Polymerase chain reaction fragments were run on native polyacrylamide gel, stained with ethidium bromide and visualized using an imager (Bio-Rad, Hercules, CA, USA).
Intensive code will now be able to be run on a local machine (with native code) while being accessed in real-time by the browser.
Samples were run on a native 4% acrylamide gel.
The samples were run on a native page gel, and half of the gel was silver-stained as previously described.
Helicase and exonuclease products were run on 10% native and 14% denaturing polyacrylamide gels, respectively, and ATPase samples were analyzed on a polyethylenimine-cellulose TLC and run in 1 M formic acid-0.8 M LiCl.
The sample was run on 15% native polyacrylamide gel at 80 V, in cool condition.
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