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Nevertheless, the patterns observed, particularly with our bias-free mapping to the N. paniculata genome, do suggest that our general conclusions may be robust to assembly and mapping differences.
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Two other genes investigated (ATCG00490 and AT1G75680) had a low expression level and were found to be robust to choice of parameter values in most assemblies.
As such, we used complete assemblies (including imputed data) for all further analyses, and we expect our conclusions to be robust to the presence of imputed nucleotides.
> -wrap-foot> The results of the experiments in Section 3 strongly suggest that our methods are robust to sequencing and assembly errors in the sequence data.
Our analyses were robust to variability in the genome assembly quality and sample preparation (Experimental Procedures and Figure S2).
Despite the fact that we used genomes at different stages of completeness, our conclusions are robust to the absence of annotation and assembly as well as potential sequencing errors.
The colloidal synthesis route is powerful to produce crystalline nanosheets, nanoribbons and nanoplatelets, and the self-assembly strategy is robust to integrate the functionalities of different nano-objects.
Assembling to a reference, when available, yields a higher quality transcriptome than de novo assembly, and this result is robust to low-levels of genomic divergence between species [ 42, 44].
While the strategies are robust to inaccuracy up to 5 mm/5° due to localization uncertainty or object displacement, they handle usual industrial assembly tolerance of far below 1mm.
However, our analysis methodology was designed to be robust, allowing for assembly errors by using non-tail-to-tail BACs, requiring the position of only one BES to be consistent with other data sets and allowing 500 kb windows for matches.
When GreenGenie2 was applied to the latest available Chlamydomonas genome assembly and the predicted v4 models were mapped back onto v3 scaffolds, GreenGenie2 appears to be robust against a seven-fold improvement in assembly continuity.
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