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Europe's return to stagnation, recession and mass unemployment cannot be reversed by adding a growth clause to the austerity treaty or issuing a few project bonds.
Notably, we found that LY294002, a specific inhibitor of PI3-kinase, effectively inhibited EGF-induced MAPK activation as well as subsequent oocyte meiotic resumption and this inhibition could not be reversed by adding additional EGF.
Some effects of vitamin deficiencies cannot be reversed by adding the vitamin to the diet, especially if damage to nonregenerative tissue (e.g., cornea of the eye, nerve tissue, calcified bone) has occurred.
In both the petri dish and mouse experiments, cyclosporine's effects could be reversed by adding antibodies to TGF-b.
Intensities of some NH signals were significantly weakened with the addition of Zn II) (Fig. 1B and 1C), which could be reversed by adding EDTA (Fig. S4).
Once the NP aggregates are initialized, subsequent nanoprecipitation will occur and their monodispersion status cannot be reversed by adding proteins (Figure 5c) because the tannic acid anions are inclined to form complexes with the buffer counter cations.
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Crosslinking was reversed by adding 20 μl 5 M NaCl (4 h, 65 °C).
In an in vitro transcription system of T. ethanolicus, RSP repressed the transcription of an alcohol dehydrogenase, whereas the repression was reversed by adding NADH.
The protein DNA complex was eluted from beads by elution buffer (1% SDS, 0.1 M NaHCO3), and cross-linking was reversed by adding in 4 μl of 5 M NaCl and incubating at 65 °C for 6 h, followed by proteinase K digestion for 2 h at 42 °C.
Furthermore, the similar phenomenon can be observed by increasing the concentration of polymerase in the reaction, as shown in Figure 5b. Figure 5 The effects of CdTe QDs on PCR were reversed by adding BSA or extra polymerase.
Cross-linking was reversed by adding NaCl to a final concentration of 0.3 mol/L and incubating overnight at 65°C in the presence of RNase A. Samples were then digested with proteinase K at 45°C for 1.5 h.
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