The partial coding sequence of LhSorTGA2 was retrieved from sequences annotated in our previous RNA-seq data.
The raw sequencing data can be retrieved from the Sequence Reads Archive (http://www.ncbi.nlm.nih.gov/sra; accession no. SRA385191).
The majority of full-length AQP transcripts could be retrieved from the sequence data alone.
All aforementioned methods have specific advantages and disadvantages, and all are limited by the amount of information that can be retrieved from a sequence at all.
However, 94% of contig sequences could be retrieved from the partial genome sequence archive of the tammar wallaby.
Sequences can be retrieved from or matched against any registered sequence source, and features retrieved from annotation sources mapped onto the alignment sequence's local coordinate frame.
Meanwhile, gene product sequences were retrieved from public sequence databases (TAIR, UniProt, Ensembl, and GenBank).
The sequence data for other species had been determined in our previous study [ 39], except for five species, for which the sequence data were retrieved from published sequence databases (Additional file 1).
For P. trichocarpa, the coding sequences were retrieved from the sequences of the P. trichocarpa sequencing project (http://www.phytozome.net/poplar).net/poplar
All sequences were retrieved from the sequenced genome deposited in CLC Genomics Workbench using reference sequences of housekeeping locus fragments (adk, atpD, fumC, gyrB, mdh, recN, infB and thdF) (http://pubmlst.org/gallibacterium/) as BLASTn queries against the genomes.
