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Additionally, Fgf20 was included in class 1. Interaction data for this gene cannot be retrieved from Mouse IDGenes, as expression of Fgf20 in the mouse neural tube starts only after E12.5 (66).
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The identified QTLs were compared with those detected by previous linkage analyses from mouse crosses which were retrieved from Mouse Genome Informatics (MGI) at http://www.informatics.jax.org.org
The results were retrieved from mouse species reference profiles.
The mouse phenotype data were retrieved from Mouse Genome Informatics (MGI) database [43].
Known protein-coding gene sets for both mouse and rat were established as follows: genomic coordinates for mouse and rat genes were retrieved from Mouse Genome Informatics (MGI) [ 45] and the Rat Genome Database (RGD) [ 46] and filtered to retain only known protein-coding genes.
At day 56, lungs were retrieved from mice and fixed in 10% formalin solution (Sigma) overnight.
Numbers of mice used in the separate experiments can be retrieved from the figure legends.
In our results, the great majority of the QTLs identified in the GWA scans overlapped with previous linkage-defined regions from mouse crosses which were retrieved from the Mouse Genome Informatics (MGI) at http://www.informatics.jax.org.org
The data for CO-related experiments on mouse models were retrieved from the Mouse Genome Informatics (MGI) database (http://www.informatics.jax.org/).org/
Essentiality of human genes was evaluated based on the data on mouse lethal phenotypes, which were retrieved from the Mouse Genome Informatics database ([ 89], www.informatics.jax.org).org
Approximately 3.0 mL was retrieved from each mouse.
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