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Unlike other conserved DNA-binding domains, Gata proteins appear to be restricted to binding consensus GATA sequences, or near variations, in DNA.
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A previous model for RB−E2F interactions suggested that RBf1 binds dE2F1 or dE2F2, whereas RBf2 is restricted to binding to dE2F2; however, we found that RBf2 targets approximately twice as many genes as RBf1.
These programs each provide searches restricted by organism and tissue type; the search results were restricted to binding sites for vertebrate factors which are either ubiquitous or neuron-specific.
In this study we asked whether the role of the LEC is restricted to binding objects with contexts or if it has a wider role in binding together other features of events.
We confirmed that our monoclonal antibodies were conformation dependent; that is, they were restricted to binding the triple-helical CII epitopes in the triple-helical state only, and bound to the rCII peptides containing their specific epitope.
The binding may be restricted to membrane-bound ganglioside GM1.
Polymorphism was found to be restricted to the DNA region implicated in binding of RNA polymerase and BvgA.
Direct ELISA was conducted by incubating hydrogels with an anti-EGF antibody for 2 h, and thus it is expected that antibody binding would be restricted to near the surface of the hydrogels due to the slow diffusion of large proteins in the hydrogels.
Additional 10- to 100-fold slower changes in thin filament activation are likely occurring during the formation of force-generating cross-bridges, but these are not triggered by Ca2+ binding and might be restricted to intermediate levels of Ca2+ activation.
These observations show that new families of sequence-specific DNA-binding proteins may be restricted to small clades and suggest that current annotations--which rely on deep conservation--underestimate the fraction of genes coding for transcriptional regulators.
However, previous results have also shown that in mini-EBVs ORC-binding appears to be restricted to DS [13], [15].
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