Interestingly, attB-adenovirus assembly and infectivity was restored by inserting a second packaging signal close to the right-end ITR, thus discarding the possibility that the attB-adenovirus genome was retained in a nuclear compartment deleterious for virus assembly.
Second, the replication capacity of NYVAC was restored by inserting two viral host range genes, K1L and C7L [38] [41]]–[41], resulting in a replication-competent but attenuated NYVAC vector (Kibler et al., submitted for publication).
The coding sequence was restored by inserting the HindIII/BstEII fragment from plasmid pVAC.FLAGαNIP-TCRζ-Not/Xba into the HindIII/BstEII digested backbone of plasmid pVAC.αNIP.TCRζ rendering the plasmid pVAC.FLAGαNIP-TCRζ.
In the absence of Htr, SR is inserted into the membrane with far less efficiency, and membrane insertion activity can be restored by the addition of the DNA sequence encoding the signal sequence peptide of BR [ 54].
If the mean TCD flow velocity dropped below 50% of the pre-clamping values and could not be restored by increasing blood pressure, or alternatively if SSEP deteriorated, an intraoperative shunt (Integra Lifesciences, Plainsboro, NJ, USA) was inserted.
It therefore could be restored by exogenous glucose treatment.
The curved coordinate can be restored by J.
This suppression could be restored by DHEA administration.
This phenotype can be restored by overexpression of sepF.
mtDNA content could be restored by reintroduction of Sucla2.
Full virulence and normal biofilms are restored by complementation experiments in which plasmids are inserted that replace the genes for the lasR-I QS into the pathogen [ 50].
