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This uncertainty could not be resolved from sequence similarity, as the pair-wise sequence identity from the duplication events marked by the blue and red arrow is 99.5% and 99.6%, respectively, highlighting the challenge in reconstructing past duplication events accurately.
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Two mutations could not be resolved: Sanger sequencing traces from both sides degrade into double peaks at the sgRNA target site.
This discrepancy has been noted since the isolation of 630Δ erm [ 18], and cannot be resolved using the sequence information from our study.
This could be resolved through sequencing mtDNA isolated from sun-protected sites, but current ethical permission for the project does not allow the recall of the skin cancer patients for this intended purpose.
Whether this correlation, which can be dubbed "Bergmann's Rule for genome size" in analogy to the "Bergmann's Rule for body size" in animals (Meiri 2010), can be disentangled from the effects of phylogeny, is to be resolved by sequencing of additional Thermotogales genomes from different environments.
However, initial studies of the Mitis group using MLSA have shown that sequence clusters corresponding to S. pneumoniae, S. pseudopneumoniae, S. mitis and S. oralis can be resolved using trees constructed from the concatenated sequences of six of the seven housekeeping genes used in the S. pneumoniae MLST scheme [ 11, 39].
These beads should not be used, as the sequences they hold cannot be resolved and they will be discarded from the final data set.
However sequences of hydrophobins from H. irregulare could not be resolved into a distinct clade.
Finally, differences between the Sanger and 454 data were resolved by examining sequence data from various strains sequenced with Illumina technology.
This was as a result of an increase in the length of the sequences obtained from cloned PCR products and the fact that haplotypes could be resolved.
Phylogenetically, these sequences appear to branch directly from the root of the C+ form, no further relationships can be resolved.
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