Since many situations cannot be resolved by technical mastery alone, adaptive leadership involves analyzing complex situations, identifying available resources and required expertise, formulating a strategy in real-time, and coordinating multiple stakeholders [15].
Equal amounts of protein from each sample were resolved by PAGE and analyzed by western blot.
Either 20 or 80% of the precipitated material was resolved by SDS-PAGE analyzedyzed by Western blotting with an anti-CD4 (H370, Santa-Cruz, CA) and polyclonal antibodies generated against the C-terminal domain of PLSCR1, respectively.
Protein concentration was estimated by the BCA assay (Pierce), and 40 µg of the total and the Triton-soluble fractions and 20 µg of the Triton-insoluble fractions were resolved by SDS PAGE and analyzed by means of Western blotting.
Initially, a cytosolic fraction and an organellar pellet (including mitochondria and lysosomes) were prepared from CCRF-CEM human cells, and their proteins were resolved by electrophoresis and analyzed for rTS protein expression (Fig. 3A).
The samples were resolved by SDS PAGE and analyzed by Western blot with anti-GAPDH, aconitase (mitochondrial matrix protein), mitofusin 1 (MFN1, outer mitochondrial membrane protein), and EGFP (for detection of polyglutamine repeats) antibodies.
The immunoprecipitated proteins were resolved by SDS-PAGE and analyzed by Western blotting.
After washing, immunoprecipitates were resolved by SDS-PAGE and analyzed by IB analysis.
The biotin-labeled proteins were resolved by SDS-PAGE and analyzed by LC-MS/MS LC-MS/MS
