Samples were resolved and quantified as previously described [37].
Samples at 150 mg/ml were induced to aggregate at 58°C for up to 36 hrs, and monomer levels were resolved and quantified by SEC-HPLC (Figure 4C).
Reaction products were resolved and quantified by infrared fluorescence (LiCor Biosciences, Lincoln, NE) using western analysis (Baker et al., 2007).
The final T cleavage reaction produced un-methylated and methylated CG containing fragments that were resolved and quantified for percent methylation via mass spectrometry.
Lipids were resolved and quantified by LC-MS/MS, as described previously [ 26], with the exception that 1250 rather than 250 pmoles C12 0 sphingomyelin (Avanti Polar Lipids, AL, USA) internal standard was added to each sample.
Sugars were resolved and quantified against standards by high-performance anion exchange chromatography with pulsed-amperometric detection (HPAEC-PAD) using a CarboPac PA20 column at 40°C, 50 mM NaOH eluent, and quadruple waveform, as recommended by the instrument manufacturer (Dionex, Sunnyvale, CA, USA).
In addition, their canonical isostructural compounds leucine, isoleucine, valine, methionine and eight other proteinogenic amino acids can be efficiently resolved and quantified.
Moreover, all four drugs were successfully resolved and quantified within a single analytical run with a short operating time (the elution time of the last peak was 6.3 min).
Under the optimized condition, the 39 species of TO-14 VOCs were well resolved and quantified in less than 3 min.
