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However, the wild type enzymatic activity could not be rescued (data not shown) and the mutant remained Endo H sensitive (Fig. 2C, right panel).
All injected animals developed the typical tra mutant phenotypes, indicating that with the RNA supplement, only the larval phenotype could be rescued (data not shown).
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In contrast, viruses with wild-type H5 HA RBS or bearing other amino acid changes at residue 228 such as G228A or G228S were rescued (data not shown).
The positive control for this experiment was Ionomycin that cause primary necrosis (Gwag et al, 1999), which can be rescued by 20 m fructose (data not shown).
The phenotype of MO5 injected embryos could not be rescued by co-injection of sept9a_tv1 mRNA (data not shown).
Both the early/slow and late/fast streaming are rescued by KHC1-975 (data not shown).
Longer extensions could not be rescued.
I didn't ask to be rescued.
She doesn't need to be rescued.
2) Be rescued.
As a control for the specificity of the Slug MO, embryos injected with Slug MO were injected with RNA encoding mycGFP-tagged Slug; both normal Sox9 expression (FIG. 1I) and craniofacial morphology (data not shown) were rescued; injection of RNA encoding myc-GFP did not rescue either phenotype (Table 1 and data not shown).
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