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Consistent with the above results, siRNA-mediated suppression of PI4KIIα expression in MIN6 cells significantly increased insulin secretion in response to stimulation with 33 mmol/L glucose (Fig. 3B), and this upregulation could not be rescued by adding PI4P, the product of PI4KIIα, to the cell culture (Fig. S3).

In addition, this property could be rescued by adding recombinant TSP-1 in the culture medium (fig. 5I).

However, cell viability can be rescued by adding purified LDL.

However, this killing effect can be rescued by adding NaPaC.

This cannot be rescued by adding the dopamine agonist pergolide (Reimer et al., 2013).

Importantly, swarming can then be rescued by adding exogenous synthetic C4-HSL, indicating that possibly the presence of SIC of tobramycin affected the production levels C4-HSL.

The notion that depletion of deoxyribonucleotides is the major effect of glutamine deprivation that leads to reduced proliferation of transformed fibroblasts, is confirmed by the fact that the proliferation defect of transformed cells is rescued by adding these precursors of DNA polymerization to low glutamine medium (Figure 6B).

Following statin treatment, tumour cells were rescued by adding mevalonate and geranylgeranyl pyrophosphate.

Attesting the specificity of these results, the effect of Dvl depletion was rescued by adding back Xenopus Dvl.

Moreover, the lovastatin-induced decrease in lipid accumulation level was rescued by adding FPP, but not zaragozic acid.

Tnfsf14 knockdown in mouse primary myoblasts also led to impaired myotube formation, which was rescued by adding recombinant sTnfsf14 protein to the media (Supplementary Figure S1).