Sentence examples for be required without adding from inspiring English sources

We used 18 cycles of PCR amplification cycles, then transferred plates immediately to 4°C (and never froze samples because extra PCR cycles may be required without adding any fresh enzyme or dNTPs).

Outside military intervention may now be required, he added.

"No co-operation with the providers is required," it adds.

A Cayley transformation is required to add flexibility to our stability solver and to allow access to specific parts of the full global spectrum which would be out of reach without such a transformation.

"Without adding the salt, we would be required to carry a liquor license," explained a ConAgra spokeswoman, Teresa Paulsen.

But vigilance is required, Mr. Fithian added.

Examination Support – Patents filed after Nov 1st will be allowed only 5 independent claims and 25 total claims without requiring added examination support, specific filing support will be required for applications with more than this number of claims.

Local service businesses can add this surcharge (or raise prices) without risking their competitive position, since their competitors will be required to take similar measures.

When low osmolarity medium was required, LB without NaCl (LB-N) was added and used [ 11].

Further investigations are required to enhance the generation of value added products (fuel) with low investments without affecting the environment.

She added: "Confidence is required.