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One of the major caveats associated with HPLC is that less routinely used chemicals like hexylamine and octane sulfonic acid sodium may be required to quantitate morphine and other opiates (Lurie & Carr 1986; Lee et al. 2005).
As multidrug resistance is an emerging threat among critically ill patients, future studies will be required to quantitate its effect in infections within this patient population.
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The field of lipidomics has yet to arrive at a general consensus as to how much accuracy is required to quantitate lipids at the level of individual molecular species.
In order to get insight into these questions histology experiments are required to quantitate macrophage infiltration, adipocyte size, markers of autophagy, ER-stress, and inflammasome activation.
The kinetic cooperativity described here for SaMTAN may extend to other MTANs, as ultrasensitive kinetic approaches are required to quantitate these catalytic site properties.
Further work on other colon cancer cell lines are required to quantitate differences in the ability of these forms of vitamin E to induce apoptosis, suppress cell proliferation and act as PPAR ligands as well as determine their effects in conjunction with other chemopreventive agents.
Hence, assays that quantitate the levels of these growth factors or other relevant biomarkers will be required to identify the appropriate patients, although assessing these biomarkers may not be straightforward.
Although specific lipids in bacteria can be quantitated, there is still a need to quantitate the total lipid content of a bacterial sample.
Ideal protein targets are required for viability across many species, are unique to prokaryotes to limit effects on the host, and have robust assays to quantitate activity and identify inhibitors.
This, however, clearly goes beyond the scope of a single paper, since it requires knowledge about pool sizes and release probabilities, which may be difficult to quantitate.
Cardiac MR can be used to quantitate the left to right shunt (Fig. 4) [42] 42].
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