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Since MIF itself is a trimer with rotational symmetry, a trimerization of Ii might be required to bind MIF [27], [46].
If the linker is relatively inert, substrate retention in the active site is enhanced if the evolving ribozyme provides at least some of the interactions that would be required to bind free (untethered) substrate.
The steroid transporters have pockets larger than what would be required to bind a simple steroid.
Alternatively, R2D2 may be required to bind specific RNA precursor molecules that PACT is unable to bind (or has a lower affinity for).
To allow for the temporal activation of the enzyme, a protein activator would also be required to bind PAD2 and "lock" calcium into the structure.
In the structure of the R2Δ complex, the substrate-binding site of Rabex-5 is partially occupied by Rabex-5CC and Rabaptin-5C212, implying that further conformational change(s) of the R2Δ complex will be required to bind Rab5.
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Since DiCN-AND noncompetitively inhibits 3β-HSD2, whash has the Gly194 and Pro195 of 3β-HSD2 in place of the Ser194 and Arg195 in 3β-HSD1, thisuggeststhathArg195195 alone in 3β-HSD1 or S194G-1 is required to bind DiCN-AND in the substrate binding site (competitive inhibition).
PSGL-1 undergoes posttranslational modifications which are required to bind selectins and are similar for binding P-selectin and L-selectin [ 11].
Results showed that the full-length TB10 is required to bind with E-Tmod, and the TB10 binding site on E-Tmod partially overlaps with the actin binding site on E-Tmod.
The Xin repeat is known to further define an actin binding domain and a minimum of 3 Xin repeats is required to bind with actin filaments [6], 8. Recently, the full-length mXinα with 15 Xin repeats was shown to bundle actin filaments [9].
The rRNA genes are transcribed by a specific transcription machinery employing RNA polymerase I (RNA pol I) with associating factors, such as UBF (Upstream binding factor) and SL1 (Selectivity factor 1); where UBF is required to bind at the enhancer region, and the SL1 complex (which contains TBP) at the promoter.
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