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We have identified the sub-cellular localization of 37 proteins shown to be required for wild type muscle structure in C. elegans by RNA interference [26], as well as another 37 proteins that have orthologs shown to be required for wild type muscle structure in flies, again by RNA interference [46].
These data suggest that SEU activity may be required for wild type levels of CYP85A2 transcript accumulation in the inflorescence.
Several cytochromes known to be required for wild type Fe(III) metabolism appeared to have been duplicated within single genomes.
More recently, Czf1 has been shown to be required for wild type adherence to silicone devices [ 45].
Only one of the nine well-conserved cytochrome families contained a cytochrome, PpcA, known to be required for wild type levels of Fe(III) reduction [ 12].
None of the cytochromes shown to be required for wild type electron transport in G. sulfurreducens were predicted to have originated from lateral gene transfer (see Additional file 9).
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The RD8b3 subregion that is homologous to SP_1345 1349 and was reported to be required for wild-type virulence in TIGR4 [65], was present in all six serotype 1 isolates in this study.
Fml1 has been previously shown to be required for wild-type resistance to interstrand cross-linking agents such as cisplatin [30].
Consistently with other studies in eukaryotes, the E3 ubiquitin ligase Rhp18 was found to be required for wild-type resistance to interstrand cross-links (supplementary Fig. 1 and [8,40 42].
We suggest that passage of sex chromosome chromatin through male meiosis may be required for wild-type function in F1 progeny, whereas disruption of Y-chromosome function through passage in the female germline likely arises because the chromosome is not adapted to the female germline environment.
On VIL-glucose bat2Δ mutant was only able to achieve 70% of the growth rate displayed by the wild type strain, suggesting that Bat2-dependent VIL catabolism was required for wild type growth, and that Bat1 was unable to compensate lack of Bat2 (Table 2; Figure 1B, CEN).
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