Certain MIC3 deletion mutants were also secreted into the parasitophorous vacuole via dense granules, supporting the view that this route constitutes the default pathway in T. gondii, and that specific signals are required for sorting to rhoptries and micronemes.
The number of subjects for RT-PCR was limited because a large number of PBMCs are required for sorting a sufficient number of CD4+CD25+CCR4+ cells for the analysis.
Certain class E VPS gene products are proposed to form three distinct endosomal sorting complexes (ESCRT-I, II, III), which are required for sorting proteins to the luminal membranes of multivesicular bodies.
Experimental work will be required to determine when PEP-CTERM proteins are expressed, where they localize, whether they are processed at the C-terminus, and whether EpsH proteins are required for sorting and/or processing.
In polarised MDCK cells the myosin VI isoform with no insert in the tail domain is required for sorting and transport of newly synthesised basolateral membrane proteins containing the tyrosine-sorting motif to the basolateral domain [47].
We therefore generated Hpo pathway follicle cell mosaics and examined the expression patterns of two key components of endocytic trafficking: Hrs (hepatocyte growth factor regulated tyrosine kinase substrate), which is required for sorting of ubiquitinated membrane proteins to late endosomes [49], and Rab7 GFP, a marker for late endosomes [50].
Interestingly, the A. thaliana AtEpsin1 protein was shown to be required for Golgi sorting of vacuolar protein (similar functions as hEpsinR and yEnt3) and AtEpsinR2 binds to PtdIns3P suggesting an endosomal sorting function (similar as yEnt3) [ 33, 34].
HRS because is well known to be required for lysosomal sorting of ubiquitinated EGFRs [24].
In the later case, TBC-2 could be required for the sorting of a critical component of yolk granules, preventing premature acidification or fusion with lysosomes.
