Interpolation without curve smoothing may be the best option, if additional degrees of freedom are required for robust modeling.
These low affinity sites conferred specificity for Ubx binding in vivo, but multiple clustered sites were required for robust expression when embryos developed in variable environments.
The performances of the control structures, which are supported with gray estimators, are given and no additional feedbacks are required for robust control action.
Immunization of mice with a synthetic nicotine nanoparticle vaccine containing TpD showed that the peptide was required for robust antibody production and resulted in a long term CD4 memory T cell recall response.
Hence, both domains of DevR are required for robust transcription activation.
Two separate E-boxes in the ADAMTS5 exonic enhancer are required for robust transcriptional activation upon myogenic differentiation.
Thus, at least one pRb protein family is required for robust differentiation even under hypoxic conditions, which rescue Rb-deficient myotube degeneration.
Previous de novo sequencing studies have suggested a minimum of 15-fold sequence coverage is required for robust identification of heterozygous SNPs [7].
Furthermore, as we have shown previously, cen1 tDNAAla is transcribed and both intact TATA and A box regions are required for robust barrier activity [16].
