Given that DDX3X seems to be required for replication of HCV and HIV, it appears that DDX3X could be a promising target for drug development against these two viruses that pose major global health threats [7].
If a real association was overestimated in the discovery sample, a much larger study sample would be required for replication.
Interestingly, the VCP-Ufd1-Npl4 pathasy has been shown to be required for replication of the intracellular pathogen Legionella pneumophila (Dorer et al., 2006).
This is an attractive hypothesis for sarDinG function as, intuitively, it seems preferable to degrade RNA, which can be replaced easily, rather than DNA, which may be required for replication restart or other DNA repair pathways.
Our observation that RuvAB did not promote CAG·CTG repeat expansion in a rep mutant (where it is known to be required for replication fork reversal) (Baharoglu et al. 2006) is not consistent with this reaction being implicated.
Additionally, conserved secondary structures are predicted among Helitrons from Lepidoptera and Diptera, which form two inverted repeats [a 5′-inverted repeat (5′-IR), and base pairing between subterminal inverted repeats (SIRs)] that may be required for replication, mobilization, or integration [ 4, 13, 18].
Ragland, R. et al. RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells.
The Center highly encourages researchers to archive their replication data, whether or not archiving is required for replication purposes.
However, experimental evidence concerning whether autophagy is required for replication of coronaviruses has been contradictory.
Of the seven mutations that differentiate iMac239 from wild-type SIVmac239, a single change (D178G) in the V1/V2 region was sufficient to confer CD4 independence in cell-cell fusion assays, although other mutations were required for replication competence.
This suggests that pVT1_88 is required for replication of pVT1.
