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In some cases where mRNA content varies greatly among cell types, external controls may be required for normalization, as proposed in microarray experiments [ 48].
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Whenever Hanoi came close to accomplishing what Vessey and others said was required for normalization, McCain says, "we kept moving the goalposts on them".
Again, no multiplications are required for normalization factor at each subband.
Furthermore, no multiplications are required for normalization factor at each subband.
Novel methods are required for normalization of small focused microarrays, and for incorporating measures of performance and quality.
Our analysis indicated that the top five genes would be required for accurate normalization of RT-qPCR data within failed and non-failed human myocardium, however, the number of genes used needs to be a balance between accuracy and practical considerations.
The pairwise variation analysis undertaken with GeNorm identified that three reference genes would be required for the adequate normalization of transcript numbers for the genes of interest within this sample set (Fig. 8, upper panel).
In summary, post-amplification normalization improves sequencing efficiency and genome assembly, and in its absence, bioinformatic normalization may be required for de novo assembly.
On the other hand, five genes are required for the normalization of the plant organ set.
This analysis was required for the normalization of the methylated DNA immunoprecipitation sequencing (MeDIP-seq) data.
The tags contain C and G nucleotides which are required for internal normalization during the Sanger sequencing [ 26].
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