The E. coli chromosome is replicated once per cell division, from a unique replication origin, oriC.
The overall 26 stimuli were replicated once entirely so a total of 52 sounds were heard.
The viral genome is maintained autonomously in proliferating cells and each viral episome is replicated once per cell cycle during S phase [1], [2].
Each experiment was replicated once, and every test was duplicated.
Each group included seven individual samples, each individual sample was replicated once (technical replicate).
All reactions were replicated once, sampled twice, and assayed twice (n = 8).
In theory, the optimum efficiency in PCR is two, meaning that every PCR product is replicated once every cycle.
Each enzyme mixture was replicated once, sampled twice, and monomeric Glc and Xyl measured twice, for a total n = 8.
All assays were replicated once, sampled twice and assayed for Glc and Xyl twice, for a total of eight replicates of each mixture.
The experiment was conducted in a randomized block design (RBD) where both 'drought' and 'control' treatments for each accession were replicated once within each of six blocks.
