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We conclude that the correlative studies must be repeated using more valid GC/MS/MS assays.
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Therefore, the experiment was repeated using more precisely controlled pores (43 nm) to achieve further vertical growth of the rod.
Because the yield began to decrease after about 20 reactions (reactions 20 22), the last reaction was repeated using more template (2 μl of the 21st reaction product instead of 0.9) and more cycles (15 instead of 7), which was enough to obtain a pure single DNA product (lane F).
Ideally, the analyses reported here would be repeated using outcomes data from more recent SUDTP patients.
The procedure can be repeated using groupings of two, three, four and more data points.
All analyses were repeated using a more recently recommended and more conservative cotinine threshold of 9.5 ng/ml [ 21] to identify non-smokers, and this did not affect the results materially.
The experiment was repeated using a more objective assessment of viability through examination of hatched larvae.
Analyses were repeated using a more liberal LD cut-off point of r > 0.7 to determine LD partners.
A modified form of an established continuity index (fraction-of-care) was used but the findings were the same when the analyses were repeated using another more widely used index (Continuity of Care; available from the authors on request).
These experiments were repeated using the more relevant human hepatocarcinoma derived HepG2 cells, since these are hepatic in origin and the liver is the primary source for cholesterol and fatty acid metabolism in humans.
To identify differential methylation associated with the highest concentrations of BPA in this cohort, the analysis described above was repeated using a more stringent cutoff for the BPA-high group (n = 5, where urinary BPA concentrations are greater than 2.60 ng/mL, the median concentration of age and gender matched individuals from NHANES 2009 2010) (Additional file 1: Table S1).
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