For example, when topsides is considered to be removed to land, it can be removed as a whole in a single lift by using huge lift vessel, or it can be removed using the reverse installation method, which removes the topsides in modules following the reverse process to its installation.
Genomic DNA was removed using DNase I. Single-stranded cDNA was synthesize from the RNA using SuperScript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA) and oligo dT primers, according to the manufacturer's instructions.
Cumulus cells were removed using hyaluronidase digestion, and single-cell zygotes at the two-pronuclei stage were recovered and immediately micro-injected cytoplasmicly with 5 10 pL of 0.6 mM antisense morpholino.
Non-paired single reads were removed using a perl script and finally the filterPCRdupl_v1.01 software [ 39] was used to remove any redundant copies from the PCR steps during library construction.
PCR duplicates were removed using SAMtools and custom scripts, and single-nucleotide variant calling was performed using a combination of SAMtools and Sidrón as described previously (Puente et al, 2011).
The single-stranded overhangs are removed using exonuclease.
Trees were removed using hand felling combined with either lop and scatter, single tree burning, or jackpot burning, which were carried out to minimize loss of shrub cover.
Following the quality screening the sequences were exported as a single FASTA file and polyA tails were removed using a custom PERL script.
Adjacent nonglioma tissue was removed using a scalpel, and tumour cells were dispersed to create a single-cell suspension.
The anti-sense RNA and single stranded circular DNA were then hybridized and abundant clones were removed using streptavidin.
