Twenty-four hours later, a FAM-labeled Aβ (200 pmol) was infused into the lateral ventricle and the plexus tissues were removed to quantify Aβ accumulation.
Gas samples were taken at 2 5 h intervals from hyphal in-growth cores, post-labelling to monitor root and mycorrhizal fungal respiration of C. Cores were left in situ until the peak respired-CO2 flux was detected, then removed to quantify non-respired-C allocation to mycorrhizal hyphae colonizing basalt.
The native enzyme is efficient for this purpose, but it contains proteins and endotoxins from V. proteolyticus that need to be removed in the manufacturing process and quantified as contaminants of the active pharmaceutical ingredient (API).
Protein lysates were centrifuged to pellet cellular debris, and the supernatant was removed and quantified using DC Protein Assay (Bio-Rad Inc .. Protein samples were processed in urea buffer and subjected to 2D-DIGE analysis (Applied Biomics , Inc Hayward, CA).
Protein extract were centrifuged at 13,000 for 10 minutes at 4°C, the supernatant was removed and quantified using the DC Protein Assay method (BioRad).
Protein extract was centrifuged at 15,115 × g for 10 minutes at 4°C, the supernatant was removed and quantified using the DC Protein Assay method (Bio-Rad, Hercules, CA).
Every 2 min, 500 μL aliquots of cell suspension were removed, cells collected by centrifugation, and the rhodamine-containing supernatant was removed and quantified by measuring absorbance at OD555.
Distilled water was used to wash it sothat excess quantify of HCl can be removed from it.
The clear supernatant was removed, quantified with DC Protein assay kit and stored at −20°C until analysis.
The membranes were removed, dried, and quantified by phosphorimaging.
