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The mixture was incubated for 1 h in a final volume of 40 µl with 50 mM Tris buffer, later partitioned using a dot blot vacuum system, so that the unbound species could be removed by three repeated washes with 250 µl of PBS.
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As before, we find that redundant interactions, albeit such that they can only be removed by two or more simultaneous deletions, explain this phenomenon (see Figure 4).
Certain restrictions on how and where fishermen may fish in EU waters could be removed by two votes on Wednesday.
Unbound dye was removed by three washes with the same buffer.
Excess avidin was removed by three consecutive wash cycles using the same citrate buffer.
Unbound protein was removed by three washes in binding buffer.
Unbound matrix molecules were removed by three washes with PBS.
Unbound secondary antibody was removed by three washes with PBS-T.
All fixative was removed by three 5-minute washes in PBS.
After binding, unbound FAM-Aβ42 was removed by three washes with cold PBS.
Proteins were removed by three rounds of phenol-chloroform-isoamylalcohol extraction.
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