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Note also that in these arguments the dependence of the coefficients on u can be removed by replacing u by u ( x ).
Optionally, the last MinElute purification step could be removed by replacing the NaOH elution with heat elution [18].
Round cells can be removed by replacing the culture medium.
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Unbound BrdU was removed by replacing the medium to DMEM.
Non-adherent cells were removed by replacing the medium after 3 days.
After 24 h, the non-adherent cells were removed by replacing the media.
After 3 days, the nonadherent cells were removed by replacing the medium with fresh complete medium.
After 3 days, the non-adherent cells were removed by replacing the spent medium with fresh medium.
After 4 h, siRNA was removed by replacing the culture medium with fresh RPMI 1640 containing 10% FBS, and cells were cultured for additional 48 72 h.
The cells were incubated for 3 days, and the nonadherent cells were removed by replacing the medium in three washing steps.
For the cells that were randomized to receive HPC, after the baseline images at time 0 had been taken, the cells were placed in an ischaemic buffer and oxygen was removed by replacing it with nitrogen gas.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com