The background scatter for each fluorescent emission spectra was removed by creating individual baseline curves from three points of the spectra, 400 nm, a baseline mid point and at 600 nm points, and solving for the exponential (y = emx).
In the last five years, N-glycosylation site occupancy has been increased from 75 85% to 99% [ 27] and undesired β-linked mannose residues have been removed by creating a P. pastoris quadruple knock-out devoid of all four endogenous β-mannosyl transferases [ 28].
The operations necessarily were unambitious and confined to collapse therapy, including thoracoplasty (removal of ribs), apicolysis (collapse of a lung apex and artificially filling the space), and phrenic crush (which paralyzed the diaphragm on the chosen side); to isolation of the area of lung to be removed by first creating pleural adhesions; and to drainage.
In both pathways, the damaged base is removed by a glycosylase, creating an abasic (AP) site.
The dead skin cells adhere to the slightly dried paste and are removed by the friction created when you gently massage it over your face.
When TFIID is experimentally removed by creating a mutation in TFIID, the valve is then "off" and mRNA output is decreased.
Realizing the effectiveness of parental VEGF-Trap may be affected by its high pI, basic amino acids were removed to create VEGF-Trap∆B1, followed by removal of additional basic amino acids to create VEGF-Trap∆B2, and finally replacing an entire protein domain with a less basic protein domain to create aflibercept (Table 2) [ 24].
The detergent was removed to create proteoliposomes by treatment with Biobeads (Bio-Rad Laboratories, HerCAles, CA) (0.1 g/mL) twice for 2 h or overnight at 4 °C.
The cell walls are removed to create spheroplasts by adding 100 μl of 25 U/μL Lyticase (Sigma), and then incubating at 30° for 45 min with rotation.
