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Hence, for each pCa-VIT plasmid, from one to three discriminating peaks can be reliably detected on the electropherogram and indicate the presence/absence of the corresponding marker, determining the qualitative composition of the DNA sample.
This removed probes whose signals were too low to be reliably detected on the array.
Comparison of ischemic myocardial segments with nonischemic segments demonstrated no significant difference in myocardial density, confirming that myocardial ischemia cannot be reliably detected on rest CCTA.
However, although the gamma peak can be reliably detected on a group-wise basis with only 70 button presses, this is insufficient to allow detection of significant gamma peaks on all individual subjects.
Both these genes' positive correlation with DC-SCRIPT expression was confirmed by RT-qPCR in the MCF7 system (Fig. 4), but both genes could not be reliably detected on the microarray platform used for the primary breast tumor specimens.
It is important to note from the outset that all radiographic assessments in each study were confined to the anterior cervical and lumbar spine vertebral corners because thoracic spine abnormalities cannot be reliably detected on radiography due to overlapping structures.
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230 miRNAs (60%) were reliably detected on the array (Supplementary Table S1) of which 35% were considered unchanged as their expression levels varied only up to 15% from untreated controls.
Transcripts that were reliably detected on at least 3 arrays that differed by a fold-ratio of >1.5 were considered to be potentially biologically important, and of these, those with a p-value of <0.05 in the CyberT test (with the parameters in Table 4) were considered to be significant.
Known genomic aberrations were reliably detected on the array and eight novel submicroscopic imbalances, likely causative for the mental retardation (MR) phenotype, were detected.
The total gene list comprised of all the genes that were reliably detected on the microarray for each accession and at each time point.
To our knowledge, this is the first study in children that demonstrates that P. jirovecii can be reliably detected using PCR on an upper respiratory tract specimen.
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