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For example, evaluating the Broad-MIT's U133A dataset (TCGA) of 173 GBM samples for genes that we identified as up regulated by MPSS, about 30% are expressed in the lower 20% percentile of the array raw intensity (data not shown) and these are known not to be reliably detected as their expression levels are close to background.
Overall this study demonstrated that at temperatures up to 24°C activity cannot be reliably detected as an independent component of the daily energy budget.
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A peptide was considered to be reliably detected at a q value ≤0.01.
We have demonstrated that an event related motor gamma band peak can be reliably detected with as few as eight subjects and a protocol lasting under 2 min.
We note, however, that mutations with a mean effect of 5% or larger can be reliably detected with sequencing coverage as low as 25× as long as the mutation does not have a large impact on the phenotypic standard deviation or relative fitness.
The detection limit (LoD), at which a low level of sIgE should be reliably detected, was estimated as the mean plus two standard deviations of the assay negative control (Table 1).
Next we tested whether glycosylation using this system can also be reliably detected by ELISA, as this method could be used for the analysis of a larger number of clones.
Butyryl-CoA levels were too low to be reliably detected in these extracts, as illustrated by the uncertainties given in Table 2 for ButCoA.
The SNCD approach, which is based on the lowest dose at which the signal can be reliably detected, warrants further development as a PoD for human health risk assessment.
If endotoxemia could be reliably detected, it may serve as an indicator of a Gram-negative infection and may direct the clinician to administer effective antibiotic therapy directed against Gram negative organisms.
We demonstrated that rare variants with minor allele frequency as low as 0.05% could be reliably detected by our method.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com