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These phantoms did not present a uniform attenuation R zf) within the range of imaging that could be reliably analyzed using Eqs.
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To validate the quality of the library and assess whether insertions due to individual transposon constructs could be reliably analyzed, we used the data obtained to identify essential genes.
We have adapted a phage-based transposition method to reliably create large and diverse transposon libraries that can be analyzed using high- throughput Illumina sequencing.
Images were then analyzed using ImageJ FIJI software.
The images were quantitatively analyzed using Image J software.
Images were analyzed using Gap Light Analyzer41.
Raw data was analyzed using Clampfit 10.4.
Data were analyzed using one-way ANOVA.
Plots were generated and analyzed using GraphPad Prism.
Data were analyzed using Prism 5.0 (GraphPad Software).
Plates were analyzed using a Molecular Devices ImageXpress High-Content Analysis SysteMolecular Devices ImageXpress High-Content
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