HMGB1 also is released readily from necrotic or damaged cells, and serves as a signal for inflammation [ 19].
Interestingly, although negatively charged BSA released readily from negatively charged collagen (col), BSA released slowly from col-coated PCL scaffolds.
These physico-chemical features suggest that after its cleavage, the LukS-PV signal peptide could readily be released from the membrane translocon context to the cytosolic or extracellular aqueous environment.
Replacement of both ion-coordinating aspartates (Asp130 and Asp159) by serine abolished transport completely, whereas mutation of Glu73 to alanine increased the activity, most likely because the substrate ion is released more readily from the binding site.
Cell-free DNA (cfDNA) is a macromolecule that can be readily detected in biological fluids and is believed to be released from either apoptotic or necrotic cells [ 9].
Of these, the former should be readily released during the alkaline incubation step, whereas the latter can normally only be released from cell walls by enzymatic destruction of the covalent linkage.
He will never be released from prison.
As pyrite is dispersed in water, ferrous iron will readily be released to solution.
Following cell damage, i-FABP is released and readily detectable in circulation [ 22].
When the Adam10 metalloproteinase was co-transfected together with Lrp4 to facilitate cleavage of the extracellular domain, Lrp4-ECD was released from the cell and became readily detectable in the culture supernatant (lane 4).
