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"I definitely thought about it," says Hoffman. "The impact will I think be relatively harsh over the next five or 10 years, starting with the fact that I think people are under the illusion that there is an easy divorce from the EU".
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The people inhabit an area where the climate is relatively harsh and transportation is costly.
The conditions of the test used were relatively harsh with extreme temperatures of −40 °C and +125 °C.
These are relatively harsh growth conditions as compared to plates rich in media (Methods).
Although this detergent is relatively harsh, the purification procedure used in this study was mild enough to retain a significant portion of F1Fo ATP synthase dimers.
In particular, our study on the virulence reaction norm with host condition (Bedhomme et al. 2004) revealed significant larval mortality effects when the environment was relatively harsh for the host, but not when the environment was relatively benign.
This pretreatment is relatively harsh, however, giving rise to the formation of unwanted and often inhibitory byproducts such as furanic aldehydes, weak acids, and phenolic compounds (Palmqvist and Hahn-Hagerdal 2000b; Thomsen et al. 2009; Klinke et al. 2004; Larsson et al. 1999).
Reductive amination has also been used in the conjugation of free reducing glycans (23) or ninhydrin-treated glycopeptides (24) to proteins; however, the reaction conditions are relatively harsh: they require large quantities of glycan and often the yields are very low.
Likewise, he felt that the tone and content of the PG-13 rated film The Dark Knight was relatively harsher to children than that of the Saints Row series due to the latter's light-hearted tone, but still noted that "as parents we know what's right and what isn't for our kids, and being aware of the content they consume is a large part of our job as parents".
Often, a three-step process is used: The first step is a relatively harsh treatment to introduce functional groups on the surface.
A drawback of this method are the relatively harsh reaction conditions under which labelling is carried out limiting the applicability of this technique, e.g. for antibodies [103].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com