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There were additional metabolites detected (see Table 4), which are likely to be reflective of cell type differences, as they were only found in the 293T cell experiments.
The striking difference between our results in the cremaster and these studies may be reflective of cell type specific, and thus organ specific, immune responses.
It is interesting to note that immunolocalisation in some of the stromal cells appears to be nuclear, although this may also be reflective of cell size.
This method is well accepted to be reflective of cell number and does not rely on measurements related to mitochondrial activity or intracellular ATP levels that could be compromised by treatments targeting ERβ which is known to be expressed in mitochondria [ 55– 55].
T cells have been shown to migrate to the lung and re-enter the circulation and as such, these cytotoxic cells identified in the peripheral blood of these patients may be reflective of cell populations in the lungs of these patients [ 18].
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DOI: http://dx.doi.org/10.7554/eLife.06498.010 Given the role of p53 in responding to cellular stress, an obvious concern would be that this signature might simply be reflective of cells undergoing cellular stress during in vitro culture.
HMGB1 is reflective of cell necrosis and activated immune cells.
To confirm that the reduction in the cell numbers was reflective of cell death, flow cytometric detection was performed using labeled annexin V (Fig. 1b).
CD24 was suggested as a marker of luminal cells in the breast [ 29], and a previous report indicated the existence of progenitor cells in luminal epithelia which could be reflective of myoepithelial cells [ 30].
This may be reflective of differences between cells derived from the healthy endometrial epithelium and those derived from adenocarcinomas or the stromal compartment and highlights the importance of using untransformed cells to model normal endometrial cell function.
Acute effects on cellular metabolism may not necessarily be reflective of the residual viable cell fraction and therefore the timing of the PET scan in relation to factors that influence the tumour cell metabolism could prove to be crucial.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com