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Briefly, a targeted biosynthetic pathway is to be reconstituted in vitro and then the contribution of cofactors, substrates and each enzyme will be analyzed systematically.
In this study we describe a skin equivalent culture model which demonstrates that normal paracrine relationships can be reconstituted in vitro and that stromal dermal fibroblasts regulate the growth of human epithelial keratinocytes.
Both systems can be reconstituted in vitro.
The circadian clock of the cyanobacterium Synechococcus elongatus can be reconstituted in vitro by three proteins, KaiA, KaiB and KaiC.
Remarkably, the clock can be reconstituted in vitro with just the three Kai proteins and ATP [2].
We have shown previously that AAV DNA replication can be reconstituted in vitro with purified proteins, including Rep, pol δ, PCNA and RFC [41], [42].
Similar(32)
At the same time, the TBhsp70-HBcAg(18 27) peptide complex was reconstituted in vitro.
Artificial adherens junctions were reconstituted in vitro by assembly of cadherin fragments at the surfaces of liposomes.
The enzymatic modification process in Prochlorococcus MIT9313 has been reconstituted in vitro, and a variety of experimental approaches have been used to try and understand how one enzyme is capable of modifying 30 different substrates.
Isolated α and β polypeptides from our most successful mutant were reconstituted in vitro with BChl a to form an LH1-type complex, which was stabilised 3-fold by calcium-ions.
This apparatus has been reconstituted in vitro [2].
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be reconstituted in mouse
be reconstructed in vitro
be assayed in vitro
be phosphorylated in vitro
be modeled in vitro
be inhibited in vitro
be simulated in vitro
be modulated in vitro
be confirmed in vitro
be generated in vitro
be expressed in vitro
be activated in vitro
be stimulated in vitro
be performed in vitro
be studied in vitro
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