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The latter enzyme is also present in MOTT (mycobacteria other than tuberculosis) and could therefore be recognized in sera from healthy individuals, exposed to MOTT.
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The NcSAG4 protein lacking the putative signal peptide at the NH2-terminus was expressed in Escherichia coli and was recognized in western blot by sera from congenitally infected cattle.
L20h-Ts3 fusion protein was recognized in Western blot as soon as 15 20 days p.i. by sera from pigs experimentally infected with 20,000 muscle larvae (ML) of T. spiralis.
Rhabdomyolysis is recognized in horses.
In sero-epidemiological surveys the most peptides were found to be recognized by sera of adults from malaria endemic countries [13] (own unpublished results).
Similarly, we and others have shown that many different antigens are phosphorylated: for instance, transient phosphorylation of serine/arginine-rich (SR) splicing family members during apoptosis leads to their association with the U1-snRNP and U3-snoRNP autoantigen complexes, and can commonly be recognized by SLE sera in a phosphorylation-dependent manner [ 22- 27].
In ELISAs with cells that had been heat killed and sonicated, all of the sera except for FtsZ, PilT-2, Adk, AcnB and HisD recognized antigens from the meningococcal cells, indicating that they were present in amounts and in a conformation that could be recognized by the sera raised against the recombinant protein.
In this case, the recombinant proteins could also be recognized by their respective sera, Atg1 and Atg2.
The purified ΔCP could be recognized by the anti-sera derived from the mice immunized by inactivated PCV2b particles.
We tested whether this novel library represents relevant antigens and can specifically be recognized by high IgG titers present in sera of sarcoidosis subjects.
The observed immunogenic antigen of M. leprae present specifically only in leprosy sera would then be recognized and analysed statistically.
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