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Our experiments investigated whether these changes in dendritic morphology could be recapitulated in a genetic model of NMDAR hypofunction, the serine racemase knockout (SR−/−) mouse.
This process can be recapitulated in a convenient organ culture system [17], [18].
2) What are the mechanisms which impede activation of neurogenesis, survival and integration into the neural network and 3) can the activation of neurogenesis following brain insult be recapitulated in a general law?
We next determined whether the TEM kinetics we had observed in patients with CLI would be recapitulated in a mouse model of severe HLI that simulates CLI in man.
Shi et al. (2012) showed that the distinct steps of human cortical development can be recapitulated in a hiPSC system, from cortical induction to the formation of functional excitatory synapses (Fig. 3).
Cells in microenvironments receive signals from many different cell types and sources, and certain pathways may only be recapitulated in a 3-D multicellular environment (Nirmalanandhan and Sittampalam, 2009; Pampaloni et al., 2009; Mazzoleni et al., 2009).
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To determine whether the above results obtained from in vitro observations could be recapitulated in an in vivo setting, we further performed the experiments with the xenograft model of human gastric cancer in nude mice (Workman et al, 2010).
Should these findings be recapitulated in an expanded clinical validation study, such an assay may be substantially more accurate and certainly more objective than the visual and optical means currently available for melanoma detection.
To distinguish between these possibilities, we first sought to test whether this negative feedback property of the SN microcircuit could be recapitulated in an in vitro preparation where extrinsic, multisynaptic sources of feedback are eliminated.
This discussion cannot be recapitulated in any detail here.
The same results were recapitulated in a transwell assay (Fig. 3c, d).
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