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By using HPLC-MS via selective ion monitoring (HPLC-MS-SIM) for M+H+ of the biotin derivatives (BMPL plus any one of BCHA, BDETA and BME), some contaminants originating from SMPP with the same m/ z as the biotin derivatives were detected, but the biotin derivatives of interest in any PMFS and those in the concentrated extract could be readily resolved from the contaminants.
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By HPLC-MS-SIM analysis of a PMFS from Mixture A via the pooling of individual compounds, the five biotin derivatives (BCHA, BBZA, BNEDA, BDEDA and BPDEDA at equal concentrations) and other detected components were readily resolved from each other.
In the absence of any added NTP, the only possible reaction outcome is the removal of the 5′-pyrophosphate, generating p*G 1 after nuclease digestion, which is readily resolved from ppp*G 1 derived from unreacted tRNA (Scheme 1).
Thus, the Abs and MCD spectroscopic features associated with the Fe(III CDO fraction can be readily resolved by removing any spectral contributions from fully reduced CDO.
We note that even though discrete fluorescence peaks may not be readily resolved, there is still important additional information from dispersive detection systems, including the fluorescence of elements of lower atomic numbers, such as phosphorus.
Using the position prediction from the KF, the problem of parameter initialization for optimization can be readily resolved.
These are issues that are unlikely to be readily resolved.
Such steric clashes can be readily resolved by the all-atom molecular dynamics simulations.
"Frankly I don't see how that issue can be readily resolved... because that's the issue that is more difficult to analyse," said SJ Berwin's Mr Holmes.
We realise that this will be a controversial matter that cannot be readily resolved.
These questions were forwarded to the developers, however, they could not be readily resolved.
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