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We found that Tar can be readily mutated to respond to new chemical signals.
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Notably, two ZFN pairs for the Sld5 gene and one for CG7224 failed, whereas both genes were readily mutated with TALENs.
In C. elegans and C. briggsae, the twitcher phenotype is associated with unc-22, a gene with more than 20 kb of open reading frame that is readily mutated in EMS screens (Benian et al. 1993).
First, methylated CpGs readily mutate to non-CpG dinucleotides.
Why do some pathogens fail to produce new antigenic variants that escape widespread immunity by the host population, whereas others readily mutate to variants that escape host immunity?
Excess unlabeled template where a TG dinucleotide upstream of the TAATTA was mutated to CC readily competed for binding of GST-Arx (Fig. 9E, lane 5), suggesting that the TAATTA site within the oligonucleotide was necessary for binding of GST-Arx.
For MFAP1, the sequence aagtgaaggtaaagcgtta was mutated to aggttaaagtgaaacgcta; for NHP2L1, the sequence ttgaaaggctcttagtcta was mutated to ttgagagactgttggtgta; and for SART1, the sequence gcaagagcatgaacgcgaa was mutated to gcaaaagtatgaatgccaa.
The conserved E53 residue was mutated to lysine in TomB.
Asp84 in SNX2 was mutated to an alanine residue to generate the cleavage-resistant mutant SNX2.
But no one knows the function of the gene that was mutated to create them.
In the porcine insulin sequence ThrB30 is mutated to Ala.
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