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These adherent cells could be readily expanded in vitro by successive cycles of trypsinization, seeding and culture every 3 days for 15 passages without visible morphologic change.
As previously reported, the hADSCs could be readily expanded in culture [ 3].
Epstein-Barr virus immortalized lymphoblastoid B cells can be readily expanded in ex vivo culture and thus be reprogrammed to pluripotency [16,17].
Especially, BMSC are identified as a relevant cell source for regeneration of focal cartilage and bone lesions, because they can be readily expanded in vitro - whereas differentiated cells, that is chondrocytes dedifferentiate upon expansion [ 7].
The ability of mesenchymal stem cells (MSCs) to differentiate into chondrocytes (in vitro and in vivo) and to be readily expanded in tissue culture without loss of multilineage potential has made them very attractive candidates for cell-based articular cartilage repair.
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mNanog HD iPS /– cells were readily expanded in culture (Fig. 6E), and expressed mNanog HD, but not the full-length transcript (Fig. 6F).
With the successful demonstration for BPA detection, the label-free DNA Y junction can be readily expanded to monitor other analytes in a simple, cost-effective, and ultrasensitive way by substituting the target-specific aptamer sequence.
These data reinforce the importance of such cells for the development of cell-based therapies for liver disease, as they provide a pool of cells that can be readily expanded, genetically manipulated and potentially used in transplantation.
Primary progenitor cells and mature T cells in PB can be readily expanded using established methods and are among the most successfully-used sources for reprogramming.
Although even small biopsies of normal urothelium can be readily expanded before undergoing senescence, this is significantly restricted in diseased tissue [29].
The existing workforce shown in Fig. 2 can be readily expanded to included community health and basic eye care workers.
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