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Cultures maintained in acyclovir for 7 days resulted in latency establishment, and virus could be reactivated using a variety of stimuli such as the removal of nerve growth factor (NGF).
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On the other hand, silenced tumour suppressor genes [ 41] could be reactivated using targeted DNA demethylation.
E-cadherin expression in cervical cancer could be reactivated using HDAC inhibitor valproic acid (VPA) suggesting that histone modification and chromatin remodeling are involved in the regulation of E-cadherin in cervical cancers [ 71].
However, attempts to show that this persistent viral RNA is still infective or can be reactivated using strong immune-suppressant drugs have failed so far (Shien et al. 2000; Forrester et al. 2003; Gall and Schirrmeier 2006; Gall et al. 2007).
Silenced transgenes were reactivated using 5-aza-2'-deoxycytidine and Trichostatin-A, and their expression was monitored by fluorescent cytometry.
BCBL-1 cells were reactivated using TPA (20 ng/ml) for the designated time.
Briefly, BCBL-1 cells were reactivated using TPA (20 ng/ml) for 16 h, before 2 h treatment with cyclohexamide (100 μg/ml).
For RT-QPCR and Western blotting experiments, EWS-FLI1 transcript was silenced and reactivated using a tetracycline inducible shEWS-FLI1 specific construct in clones derived from the A673 cell lines.
In the UK, according to Mastaglio, there is a "gold standard" that means it is impossible for a firearm to be reactivated and used.
Therefore, to prevent inhibition of ORF57 protein production, cells were concurrently reactivated using TPA and either mock transfected or transfected with scramble or PYM-specific siRNA, which allowed sufficient ORF57 protein to be produced before optimal PYM depletion.
AChE enzyme was successfully reactivated using 2-pyridine aldoxime methiodide.
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