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Furthermore, each CV experiment can be randomly replicated a number of times in order to increase the stability of model evaluation (but we did not do that in the present study).
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Each disturbance treatment was randomly replicated twice in each block resulting in 24 replicates per region (4 treatments×2 replicates×3 blocks = 24 plates) (Fig. 1B).
Each level was randomly replicated thrice.
Each cross-validation experiment was randomly replicated eight times.
Nine lactating ewes were randomly assigned to a replicated (n = 3) 3 × 3 Latin square design.
Eight ruminally cannulated steers (366 ± 32.2 kg initial body weight [BW]) were randomly assigned to a replicated 4 × 4 Latin square design.
Six rumen-fistulated, lactating Holstein dairy cows (627 ± 16.9 kg of body weight, 180 �� 6 d in milk; mean ± standard deviation) were randomly assigned to a replicated 3 × 3 Latin square design trial.
Eight multiparous Holstein cows, 114 ± 14 d pregnant and 685 ± 39 kg of body weight, were randomly assigned to a replicated 4 × 4 Latin square design with 14-d periods.
Six ruminally cannulated, multiparous Holstein cows (67 ± 9 d in milk at trial initiation) were randomly assigned to a replicated 3 × 3 Latin square design with 14-d periods; the first 11 d of each period were for diet adaptation followed by 3 d of sampling and data collection.
A total of 84 pots (3 harvests (see below) ×7 treatments ×4 replicates) were randomly arranged in a polytunnel that received natural light in the Experimental Garden of Fudan University based in Shanghai, China (31° 18 ' N; 121° 30 ' E) in early April , 2008
Approximately 50 berries were randomly selected from a 1000-berry biological replicate for RNA extraction.
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