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Building upon these earlier attempts, in the present work we improved transformation conditions through the development of two new vectors and also gained insight into the fate of the transferred T-DNA, which was found to be randomly integrated, with a preference for repeat element-containing genome sites.
Similar(59)
These insertion sites suggest that the transgenes were randomly integrated in the chromosomes.
These samples were randomly integrated into several runs including all steps from pre- to postanalytics to include all influencing factors.
The Xi is bearing a randomly integrated CAG-driven GFP allele near the centromere [ 58 ].
The transgenes are randomly but permanently integrated into the host genome at multiple sites together with viral vector backbone.
SVs were randomly simulated and integrated into the source sequence in a non-overlapping manner.
Furthermore, the integrity of randomly integrated transgenes is not guaranteed, which is complicated by the fact that transgenes often integrate as concatemers containing varying numbers of transgene copies.
Critically, planning should be integrated with transport.
They can be integrated with other systems.
They can be integrated with various materials.
And can it be integrated with reality?
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