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The percentage of chimerism could be quantitated either by agarose gel analysis or Southern blot analysis.
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Incubation was carried out for 2 h at room temperature and development was by enhanced chemiluminescence (Amersham UK). Loading was quantitated either by β-tubulin or by a parallel gel, which was stained with Coomassie brilliant blue.
The fluorophor can be quantitated by fluorescence intensity measurement.
Real-time PCR products may be quantitated by generating a standard curve, or quantitated relatively to a control gene.
Can this be quantitated?
This should be quantitated.
Library DNA was quantitated using either a Nanodrop or a BioAnalyzer and sequenced on an Illumina GA2 by the DNA Technologies Core Facility at the University of California-Davis (http://genomecenter.ucdavis.edu/dna_technologies/) or at the University of British Columbia Genome Science Center.
Skin injury by either agent alone was quantitated by the probability of tail necrosis as a function of dose of agent.
Extracted RNA was quantitated by spectrophotometry and quality evaluated by running samples on denaturing agarose gels.
Following extraction, proteins were quantitated by BCA protein assay and resolved by SDS-PAGE.
Cell viability was quantitated spectrophotometrically by measuring the optical density at 450 nm, with a reference wavelength of 650 nm.
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