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All LCM-captured samples yielded sufficient total RNA (10 – 20 μg) that could be quantitated by the low-range Ribogreen RNA Quantitation Kit (Molecular Probes).
Incubation of THP-1 cells at 37 °C with fluorescence-labeled platelets and anti-platelet antibody promoted ingestion of platelets that could be quantitated by flow cytometry.
Since individual choline compounds and phospholipid classes cannot be quantitated by in vivo MRS, we determined their (absolute) tissue levels by 1H MRS of whole-brain extracts (Fig. 1).
Various β2 splice variants, such as β2a, have the ability to dramatically slow inactivation of α12.x currents [19], [20], which can be quantitated by measuring the residual current after 350 ms of depolarization and normalizing it to the peak current amplitude (R350; Fig. 2C,D).
The fluorophor can be quantitated by fluorescence intensity measurement.
Apoptotic cells can be quantitated by light microscopy, and an apoptotic index can be calculated.
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Scanned images were quantitated by Illumina Beadscan, v3.
In addition, trypan blue (TB) was quantitated by light microscopy.
Reads were quantitated by counting the number of reads across exons.
Top, COX-2 mRNA was quantitated by real-time PCR using GAPDH as internal control.
Infected cells were quantitated by fluorescence microscopy and automated image analysis.
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