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Their effectiveness and importance cannot be overstated, but, unfortunately, cannot be quantitated as typical aspects of an intervention.
Flow-mediated, endothelium-dependent dilation (FMD) can be quantitated as an index of vasomotor function, and the noninvasive nature of the technique permits repeated measurements over time to evaluate the effectiveness of interventions that may affect vascular health.
Alternatively, for the DILPs, the levels in the median neurosecretory (mNSCs) brain cells that secrete them can be quantitated as a reduction in circulating DILPs – this usually correlates with an increase in DILPs in the mNSCs.
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The use of MS detection also adds a level of confidence in the identity of the species being quantitated, as well as indicating peaks that result from overlapping glycans, where the quantitative results will be suspect.
Depletion of the corresponding transcript after dsRNA treatment was verified using rtPCR (Figure 6A), and cells were pulsed with Fdex and A568Tf and their normalized per cell uptake was quantitated as before.
S1P and total ceramide were quantitated as described.
Only the nuclear staining was quantitated, as the software did not permit simultaneous quantitation of membranous, cytoplasmic and nuclear staining based on differences in intensity of positive brown staining.
The structures were quantitated as in panel (b).
(b) Changes in the size of acinar structures were quantitated as in Figure 2b.
Histologic results were quantitated as an indicator or myogenic differentiation levels.
Membranes from Sf9 or HEK293 cells expressing 6hNETBR or its mutants were prepared, and the expressed ETB receptors were quantitated as described previously62,63.
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