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Bacterial community diversity can be quantified using phylogenetic approaches applied to shotgun metagenomic data.
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Further, the influence of phylogenetic resolution on metrics of phylogenetic dispersion has not been quantified and a priori predictions are less clear because phylogenetic dispersion is quantified using randomizations that may mitigate the loss of statistical power and the potential to over- or under-estimate phylogenetic diversity and dispersion.
To quantify phylogenetic informativeness (PI) for each dataset, site-specific rates and informativeness profiles were quantified using the program HyPhy in the PhyDesign web interface [ 136].
Proteins were quantified using Qubit® 2.0 Fluorometer.
Fluorescence was quantified using ImageJ.
Immunoblots were quantified using ImageJ.
Total RNA was quantified using Qbit.
RNA samples were quantified using spectrophotometry.
Protein content was quantified using EZQuant (Invitrogen).
RNA was quantified using ultraviolet spectrophotometry.
Cell size was quantified using SigmaScan Pro.
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