Reaction parameters were: 10 min polymerase activation, followed by 40 cycles of 95°C for 15 s and 57°C for 1 min. Gene expression was quantified separately for each cDNA sample.
The KNOB was quantified separately for the left and right hemispheres, in the axial (transverse) plane on each spatially aligned brain (see Figure 4) following procedures previously used in human and chimpanzees brain specimens ([11], [24].
Lunge count for 'between condition' assays (control vs SD) was quantified separately for each fly of the designated condition; lunge count 'within condition' was quantified as the combined number of lunges from both flies in the assay.
This deactivation effect was quantified separately for each focus condition in each of the 6 FG ROIs designed to segregate posterior, mid-, anteriorrior fusiform regions within each hemisphere (Table 3).
Using a non-linear optimization procedure applied to the experimental data, the polarisation resistances at anode and cathode can be quantified separately, which allows to obtain p⊕ values that are corrected for the electrode polarisation.
The total tax expense or income is the sum of these two items, which should nonetheless be quantified separately.
The two sub-processes that lead to fatigue crack extension were quantified separately in a recent model for small fatigue crack growth applicable to engineering alloys.
Remaining metabolites, including selected glucuronides, Tam- N-Oxide, 4′-OH-Tam and 4′-OH-NDM-Tam derivatives, as well as selected (E)- and (Z -isomers, have been quantified separately (see Additional file 2 for the examples of sample calibration chromatograms).
Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay.
Left and right striatal uptake was quantified separately.
