Results for 26 samples were below the detection limit of the thermal-block PCR but could be quantified by real-time (LightCycler) PCR.
For example, electrophoresis confirms the presence of the gene that can be quantified by real-time PCR and further deciphered by sequencing.
This method allows RCA to be quantified by real-time quantitative PCR using primers and a probe designed for E1 DNA.
Expression levels of a panel of nine cumulus markers of oocyte competence as aforementioned will be quantified by real-time PCR in cumulus cells removed from one cumulus oocyte complex (COC /patient.
Samples will be reverse transcribed using the Taqman®MicroRNA Reverse Transcription Kit (Applied Biosystems, Life Technologies, Carlsbad, California, US) and mature miRNAs will be quantified by real-time PCR using the Taqman®MicroRNA Assays [ 26]. miRNA expression will be analyzed using the BioMark 96 96 Dynamic Array (Fluidigm Corporation, San Francisco, California, US).
Gene expression was quantified by real time PCR on an ABI StepOne Plus machine (Applied Biosytems).
Purified monocytes were isolated by FACS sorting, and HIV DNA and RNA levels were quantified by real time PCR.
Total RNA was extracted from the sorted cells and the expression of different cytokines was quantified by Real Time PCR.
Vero cells were used for viral culture, and HSV-2 DNA was quantified by real time PCR, as described [54].
Expression of Kir2.1 in chicken embryos was quantified by real time PCR using E8 ventral spinal cords.
The amount of PDGFRβ mRNA was quantified by real time PCR using Taq primer (Hs00387364, Applied Biosystems, Switzerland).
