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The numbers of circulating CD133+ HSC and circulating CD34+ HSC in peripheral blood will be quantified by flow cytometry according to ISHAGE protocols.
Consequently the fraction of undifferentiated ESCs in a population can be quantified by flow cytometric analysis for GFP (Wray et al., 2011).
This inhibits any further caspase activity and retains FLICA intracellularly so that active caspase-8 molecules can be quantified by flow cytometry.
Since CD8+ T cells recognize antigen in the form of short peptides bound to Class I molecules, cells with the appropriate T cell receptor will bind to the labeled tetramers and can be quantified by flow cytometry.
The externalization of PS was an early event of apoptosis; thus, by using annexin V, conjugated with a fluorochrome (FITC), the event of apoptosis could be quantified by flow cytometer.
Early EPC characterised by the expression of CD34, CD133 and the VEGFR-2 (Flk-1, KDR) are present in the circulation at very low numbers and cannot be quantified by flow cytometry without previous enrichment procedure.
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Thus, AO fluorescence intensity, which represents AVOs formation, can be quantified by flow-cytometry.
Endothelial dysfunction is an early step in the atherosclerotic process and can be quantified by flow-mediated vasodilation (FMD).
ΔΨm was quantified by flow cytometry analysis using a FACScalibur flow cytometer, as described57.
At each point samples were stained with PI, and incorporation was quantified by flow cytometry.
GFP-positive cells were quantified by flow cytometry at 24 h p.i. Data are mean ± s.d.
More suggestions(16)
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